Down-modulation of cancer targets using locked nucleic acid (LNA)-based antisense oligonucleotides without transfection
نویسندگان
چکیده
منابع مشابه
In vivo tumor growth inhibition and biodistribution studies of locked nucleic acid (LNA) antisense oligonucleotides.
Locked nucleic acids (LNA) are novel high-affinity DNA analogs that can be used as genotype-specific drugs. The LNA oligonucleotides (LNA PO ODNs) are very stable in vitro and in vivo without the need for a phosphorothiolated backbone. In this study we tested the biological fate and the efficacy in tumor growth inhibition of antisense oligonucleotides directed against the gene of the large subu...
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For the past 15-20 years, the intracellular delivery and silencing activity of oligodeoxynucleotides have been essentially completely dependent on the use of a delivery technology (e.g. lipofection). We have developed a method (called 'gymnosis') that does not require the use of any transfection reagent or any additives to serum whatsoever, but rather takes advantage of the normal growth proper...
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Nucleic acids are becoming increasingly important as therapeutic molecules mainly for the ease with which specificity for a vast range of targets of these drugs is achieved. Yet the dose rates of several mg/kg are not easy to provide. Reform of drug policy has been suggested earlier to facilitate newer therapeutic strategies to be put to use. With the current technology, each mg of the unmodifi...
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Hybrid nucleotide-lipids containing locked nucleic acid (LNA) show enhanced hybridization properties with complementary single strand RNAs compared to DNA lipid analogues. The LNA adenosine lipid features unique binding properties with a high binding affinity for poly-uridine and the entropically driven formation of a stable complex (K(d) ≈ 43 nM). Enhanced hybridization properties of LNA-based...
متن کاملDesign of antisense oligonucleotides stabilized by locked nucleic acids.
The design of antisense oligonucleotides containing locked nucleic acids (LNA) was optimized and compared to intensively studied DNA oligonucleotides, phosphorothioates and 2'-O-methyl gapmers. In contradiction to the literature, a stretch of seven or eight DNA monomers in the center of a chimeric DNA/LNA oligonucleotide is necessary for full activation of RNase H to cleave the target RNA. For ...
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ژورنال
عنوان ژورنال: Gene Therapy
سال: 2010
ISSN: 0969-7128,1476-5462
DOI: 10.1038/gt.2010.133